The course in biophotonics provides an introduction to optical phenomena encountered when measuring on biological samples and introduces optical diagnostic methods and instrumentation employed for evaluating the state of biological tissue and organic matter. Methods include: Remote sensing techniques for studies of biological flora and fauna. Practical laser spectroscopy based on light emitting diodes for measurements of absorption and fluorescence. Polarization techniques for analysis of light scattered from biological samples. Furthermore, the course includes imaging and microscopy techniques for investigations of small structures. Moreover, aspects of data analysis are treated. A particular emphasis is put on projects letting student groups build their own instrumentation for a variety of case studies. The projects will be oriented around the combination of an instrumental approach with a biological sample and will include aspects of optoelectronics, optomechanical construction, acquisition, measurements, data interpretation and presentation. Two other laboratory exercises deal with polarization of light scattered from biological samples, spectral imaging and microscopy. Additional insights in biophotonic diagnostics are gained from visits to laboratories of research partners at Lund University and related local industry.

Administrativ info

Course codeFBRN10
Period: VT2 (LP4)
Credits: 7,5 Hp
Responsible: Mikkel Brydegaard och Christian Brackmann
Introduction: 23/3 kl. 13.15, in Hall Rydberg, H418

Course syllabus -

Contents and outline

The course covers underlying physical principles of light in biology with a tour of biophotonic instrumental approaches developed until today. This knowledge forms a base for selection and development of appropriate techniques and instrumentation for probing key features to characterize, classify or grade the health/quality of biological samples. For deepened understanding and practical experience, the course offers a creative hands-on project for student groups to freely develop a simple setup for a biophotonic technique, apply it to a selected sample and present it at the end of the course. The course is arranged with 14 lectures, visits to other labs, and 3 laboratory exercises, of which one is the course project. To pass the course as a whole, the student needs to pass the laboratory exercises and the written exam. The final grade is set by the result achieved on the written exam.